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Objective To study the effect of the icariin on apoptosis and cytoskeleton of osteoblasts in response to overload damage. Methods The four-point bending loading device was used to simulate the mechanical environment of overload damage and establish the cell overload damage model. According to whether the drugs were added before or after mechanical loading, the experiment was divided into blank control group, icariin group, damage group, damage prevention group and damage treatment group. Cell apoptosis was detected by flow cytometry. The specific fluorescent dyes were used to label the actin filament and the nucleus, and the changes of cytoskeleton were observed under laser scanning confocal microscope. Results Compared with control group, the apoptosis rate of damage group was the highest, and the icariin group was the lowest (P<0.05). Compared with damage group, the apoptosis rate of the damage prevention group was the lowest (P<0.05). The damage group showed cell shrinkage deformation, microfilaments disorganization, loosely arranged skeleton with vague outline, even broken skeleton. The morphological changes of cytoskeleton in damage prevention group were not significant, and there was no obvious change in cell nucleus. Conclusions Icariin can inhibit the apoptosis of osteoblasts after overload injury and maintain the stability of cytoskeleton to some extent.
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?AlM: To evaluate the expressions and significances of endoplasmic reticulum stress related protein ( BlP ) , hypoxia-inducible factor-1α ( HlF-1α) and vascular endothelial growth factor ( VEGF) in the retina of diabetic rats. ?METHODS:Seventy-two male Sprague-Dawley ( SD) rats were chosen and divided randomly into 6 groups:normal control 2mo (C2m, n = 12), diabetes mellitus 2mo (D2m, n=12), normal control 4mo (C4m, n=12), diabetes mellitus 4mo (D4m, n = 12), normal control 6mo (C6m, n=12) and diabetes mellitus 6mo (D6m, n=12 ) . The diabetes mellitus mouse were induced by intraperitoneal injection of 0. 1mol/L streptozotocin (STZ, 65mg/kg). The expression of BlP, HlF-1α and VEGF in the retina were detected by enzyme- linked immuno sorbent assay. The location of BlP, HlF-1α and VEGF in epiretinal membranes were investigated by immunohistochemistry staining. ?RESULTS: BlP were significantly increase than control groups in all DM groups with the course of diabetes ( P ?CONCLUSlON:The expressions of BlP, HlF-1α and VEGF increase in the retina of diabetic rats than control groups with progressive of diabetes mellitus, both endoplasmic reticulum stress and HlF-1α signal path play important role in the progress of diabetic retinopathy.
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Objective To study mechanical properties of the medical water-jet scalpel when cutting parenchyma such as liver and verify its tissue-selective cutting characteristic. Methods The tension mechanical properties of porcine liver parenchyma and its vessels with different sizes were determined. Porcine and Wistar rat liver tissues were cut with arteriovenous vessels well reserved, and pathological section of the rats were analyzed by HE staining to explain the experimental phenomena. Results When the working pressure was set at 3 MPa, the incising and separating on the right lobe of porcine liver by medical water-jet scalpel in this experiment were done with minimal vessels of 0.8 mm in diameter left. Pathological sections from ordinary scalpel and medical water-jet scalpel showed that the medical water-jet scalpel caused smaller tissue damage. Conclusions The medical water-jet scalpel could cut heterogeneity soft tissue with highly-selective characteristics, which may effectively avoid the existing “one size fits all” phenomenon caused by ordinary scalpel.
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Objective To investigate the effect of mechanical loading with different magnitudes on the proliferation, differentiation and activity of preosteoclasts and osteoclasts. Methods One group of RAW264.7 preosteoclastic cells cultured in osteoclast inductive medium were subjected to the cyclic tensile strain for three days, and then cultured for four days; the other group of RAW264.7 cells were induced in osteoclast inductive medium for four days to be osteoclasts, then subjected to the cyclic tensile strain for three days. Results Under the tensile strain at different magnitudes, the proliferation variations in two groups of RAW264.7 cells were approximately identical, but changes in the activities of tartrate-resistant acid phosphatage (TRAP) and numbers of TRAP-positive multinucleated cells (osteoclasts) in the two groups were significantly different. Under the moderate tensile strain (2 500 με), the reduction of TRAP activity and osteoclasts number were both the highest in the first group, and both the lowest in the second group. Conclusions The influence of different tensile strain on osteoclast differentiation and osteoclastic activity of preosteoclasts in early differentiation is different to that of the preosteoclasts already differentiated into osteoclasts.
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BackgroundDiabetic retinopathy (DR) is the result of the cytokine network disorders,the imbalance of angiogenic factor and vascular inhibitory factor is the start factor.ObjectiveTo analyze the levels of CXC chemotatic factors of type 2 diabetes mellitus patients,evaluate the clinical application value of them in different clinical types of DR using receiver operating characteristic (ROC)analysis and to approach the new way of individualized treatment.Methods This was a prospective research.The gold standard was ophthalmolscope and fundus fluorescein angiography.The levels of CXC chemotatic factors and multiplicaiton factors were measured in 96 cases with type 2 diabetes mellitus (66 cases with retinopathy and 30 cases without retinopathy as control).The assessment tasks were performed for these index and courses of DR with ROC curve.Results The expression of age,course of disease has significant difference in different courses of DR ( F =8.507,P =0.001 ; F =28.143,P =0.000).Compared with the control group,the expression of growth-related oncogene-α ( GROα ) ( t =- 2.172,P =0.035,AUC =0.625 ),whole blood viscosity 200 ( t =- 3.724,P =0.001,AUC =0.904 ) and neutrophilic leukocyte (t=-2.562,P =0.013,AUC =0.577 ) has significant difference in the group of mild NPDR.Compared with the control group,the expression of interferon-γ-inducible protein 10 ( IP-10 ) ( t =-3.591,P =0.001,AUC =0.592 ),platelet derivation growth factor-BB ( PDGF-BB ) ( t =- 3.233,P =0.003,AUC =0.735 ),vascular endothelial growth factor(VEGF) ( t =- 3.617,P =0.001,AUC =0.776 ),C peptide ( t =- 3.366,P =0.002,AUC =0.962 ),leukocyte ( t=-3.201,P =0.003,AUC =0.852) and neutrophilic leukocyte(t =-4.201,P=0.000,AUC =0.852) has significant difference in the group of moderate and severe NPDR.ConclusionsCXC chemotatic factors may act as reactivator in the pathogenesis of DR,GROα and IP-10 may be useful for clinical monitoring of the severity of DR,and evaluating the imbalance state of chemotatic factors maybe a new approach to clinical monitoring and prognosis of DR.
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Objective To design and build a new dynamic load and circulating perfusion bioreactor system and test its performance. Methods The design principle of the bioreactor system was specified and the dynamic strain loading system and 3D perfusion culture system were designed and built accordingly. A special culture chamber for 3D perfusion and compressive loading was also developed. The sterility of the culture chamber and the accuracy and stability of the strain loading were measured, and the result from the culture of tissue engineering bone was preliminarily observed. Results This bioreactor system could provide compressive strains with different magnitudes and frequencies, as well as perfusions under different flow conditions. It could be controlled accurately and operated easily with a steady performance. No germs were grown in the culture medium after 5 days’ running. The preliminary results showed that after the tissue engineering bones were cultured in the bioreactor for 10 days, cell proliferation and ALP activity in this perfusion culture and loading group were significantly higher than those in the static culture group and the simple perfusion culture group. Conclusions The bioreactor could be an ideal dynamic culture and loading device for biomechanical study of tissue engineering bone.
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Objective To solve the danger and difficulty in transferring seriously injured victims. Methods The operating principle, construction design, electronic control system and software program flowchart of a robot transfer arm for victim-transfer were introduced.Results and Conclusion The victim didn not have to change their body posture during transfer. The procedure is very simple.A push at only one key is enough,without secondary injury.
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Objective To determine serum cytokine profiles and levels in patients with early diabetic retinopathy(DR)by using antibody array technology and analyze their clinical significances.Methods Among 32 patients with type 2 diabetes mellitus,16 patients with mild to moderate non-proliferative DR were as DR group;and 16 patients without retinopathy,as diabetic control group.Eight healthy subjects were selected as normal control group.The profiles of 42 cytokines were detected by human cytokines antibody array(Raybiotech).Results Compared with diabetic control group,in DR group,levels of epithelial neutrophil-activating peptide-78(ENA-78),growth related gene(GRO),regulated upon activation normal T cell expressed and secreted(RANTES),angiogenin,vascular endothelial growth factor(VEGF)and platelet-derived growth factor(PDGF)increased significantly(P
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0.05),while there were significant differences in VEGF mRNA expression among the rest of groups(P
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Objective To evaluate the clinical effects of emergency neoplasty on corneal penetrating injury. Methods A total of 102 cases of corneal penetrating injury were investigated.The patients underwent micro-trauma technique suture,optical iridectomy,extraction of anterior segment foreign bodies,nepheloid lens removal,and artificial lens implantation.The vision of pre-and post-surgery was compared and relationship between injury location and recovery was analyzed. Results The injury of cornea repaired completely 1 month after surgery.Cross infection was not found in the patients with one-year long follow-up(80% of all patients).The vision of all patients raised.The injury in center and nasal side affected vision significantly. Conclusion Timely surgery to corneal penetrating injury may be considered in order to improve the recovery of the traumatic eyes.